NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN
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NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN
The NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN is a sample amplification kit for whole genome sequencing of mpox virus (MPXV) in biological samples. The kit contains 88 primer oligonucleotide pairs in two premixed pools (Pool 1 and Pool 2), each directed at a unique 2.5 kB stretch of the MPXV genome, covering ≥ 99.9 %. With the NextGenPCR™ Arctic Fox HF Chemistry polymerase mix and our recommended cycling protocol, amplification of the MPXV genome can be completed in ≤ 45 minutes total cycling time. Reagents are provided for a total of 96 samples.
The MPXV Amplification Primer Panel – containing all primer sets – can be purchased separately (cat. #50025-1, available from Molecular Biology Systems) if a custom PCR setup is required.
Note: The NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN does not include the reagents or the instrumentation necessary to sequence amplified DNA products. These materials must be purchased separately from another vendor.
SPECIFICATIONS
Catalogue number: 50027-1
The NextGenPCR™ MPXV library preparation protocol was tested on 40 samples in close collaboration with Matthijs Welkers and Marcel Jonges from the Dept. of Medical Microbiology & Infection Prevention at Amsterdam UMC (location AMC), The Netherlands.
Using NextGenPCR™ MPXV library preparation up to 20 samples could be multiplexed in a single nanopore flowcell with greatly improved sequencing depth and coverage owing to its PCR tiling based target enrichment. This additionally results in lower costs per sample. The NextGenPCR™ MPXV library preparation method was successfully employed to sequence the first known case of MPXV infection in The Netherlands.
For more details, see also our NextGenPCR™ MPXV Amplification Primer Panel (96 reactions)
