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Ultrafast r16S PCR Nanopore Sequencing for Immediate Results

Ultrafast 16S full gene amplification with NextGenPCR™

Revolutionizing 16S rRNA Analysis with NextGenPCR™

The NextGenPCR® 16S Full Gene Amplification Kit is a complete PCR assay solution for ultrafast bacterial composition analysis. The kit includes everything you need: primer oligonucleotides, ready-to-use polymerase master mix, and a genomic bacterial DNA control. With a total cycling time of ≤ 21 minutes, this kit allows users to amplify the full-length 16S rDNA gene, making it possible to complete a full PCR Nanopore sequencing analysis in just a single working day.

Why NextGenPCR™?

NextGenPCR™ offers the fastest solution for 16S amplification available today, significantly reducing the time required for traditional PCR steps. By reducing the amplification time to under 21 minutes, microbiome researchers can accelerate the full workflow, from PCR to sequencing and analysis, effectively cutting the time to result and ensuring real-time insights into microbial communities. This speed is invaluable in fields ranging from clinical diagnostics to environmental microbiome monitoring.

Key Components and Benefits of the NextGenPCR® 16S Full Gene Amplification Kit:

  • Primer Oligonucleotides: Carefully designed to target the full 16S rRNA gene, ensuring optimal amplification of a broad range of bacterial species.
  • Ready-to-Use Polymerase Master Mix: Minimizes setup time and reduces the risk of contamination, enhancing the reliability and reproducibility of results.
  • Genomic Bacterial DNA Control: Ensures that every run is validated, providing confidence in your experimental outcomes.

1. From PCR to Sequencing in One Working Day

Complete Workflow with Nanopore Sequencing
Once amplified with NextGenPCR™, the full-length 16S rDNA can be rapidly analyzed using Nanopore sequencing. The combined approach of ultrafast PCR with real-time sequencing capabilities allows for:

  • Comprehensive Profiling: Full-length sequencing provides a more complete picture of microbial diversity compared to partial gene analysis.
  • Rapid Turnaround: Complete the entire process from amplification to data analysis within 24 hours, providing a faster route to actionable results.

2. Advantages for Microbiome Research

Unprecedented Speed for 16S Analysis
Traditional sequencing workflows can be time-consuming, often requiring multiple days to complete all steps from DNA extraction to sequencing. NextGenPCR™ removes this bottleneck, reducing amplification times to mere minutes while maintaining high fidelity in the reaction. The integration with Nanopore sequencing further ensures that long-read, real-time data acquisition is available at the same unprecedented speed.

Versatility Across Sequencing Platforms
The kit is designed to work seamlessly with Nanopore, but also integrates effectively with other sequencing platforms such as Illumina. This versatility means that laboratories with varied infrastructure can benefit from the speed of NextGenPCR™ without the need for significant changes to their existing workflow.

 

3. Comprehensive Solution for Laboratories

NextGenPCR™ and Sequencing Compatibility
Whether you’re working with Nanopore, Illumina, or another platform, NextGenPCR™ provides unmatched flexibility to fit within your existing infrastructure. Its compatibility and ease of integration mean that labs can quickly adopt this technology and start benefiting from faster workflows immediately.

Minimal Setup, Maximum Efficiency
The ready-to-use reagents and validated control materials ensure minimal hands-on time while maintaining rigorous standards for quality and reproducibility. This means that your lab can spend less time on setup and more time obtaining valuable data.

 

More information? Download Application Note

Download the 16S application note.

 

 

 

 

 

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With results available within a single working day, NextGenPCR™ opens up new possibilities in clinical, environmental, and industrial microbiology. Check out the latest innovations in rapid PCR technology using NextGenPCR™ cycling:

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To inquire about NextGenPCR® 16S Application Kit, please contact us via the contact form below.

 

Revolutionizing 16S rRNA Analysis with NextGenPCR™